Figure 8. Rapamycin treatment had no significant effect on age-related changes in maximal O₂ consumption and body temperature, but increased RER.

(A–C) Findings from a metabolic assessment (indirect calorimetry) of the 25-month cohort (young control, n = 10; vehicle, n = 10; rapamycin, n = 14). (A) Body temperature. (B) Maximal O₂ consumption. (C) Average RER. Data were analyzed by fitting them with a linear model against the factors of age (young vs. old), treatment (rapamycin vs. vehicle), and body weight. (D) Effects of rapamycin on RER in young mice (control, n = 9; rapamycin, n = 8). Whisker plots display minimum, 25th percentile, median, 75th percentile, and maximum. P values and fit coefficients with 95% confidence intervals are shown; statistically significant differences (P < 0.05) are denoted by bold font. See Supplemental Tables 11 and 12 for complete findings.