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. 2013 Jul 25;123(8):3624–3633. doi: 10.1172/JCI69746

Figure 4. Ectopic expression of DN-GNAS increased mineralization of adipose-derived stromal cells.

Figure 4

(A) RT-PCR analysis of PPARG and ALPL in adipose-derived stromal cells after culturing for 5, 7, and 14 days in DMEM with 10% FBS. Data represent mean ± SD. (B) ALPL activity assay in adipose-derived stromal cells after 5 days of culture. (C) Alizarin red staining of adipose-derived stromal cells after 14 days of culture. Original magnification, ×10 (B and C).