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. 2001 May 1;29(9):1951–1959. doi: 10.1093/nar/29.9.1951

Figure 8.

Figure 8

(A) HPLC separation of the polar oligonucleotide adducts. Following large scale synthesis and isolation of the C8-dG-PhIP adduct, fractions containing the polar adducts were combined and separated on a semi-preparative column using a sodium phosphate to methanol gradient (0–27% over 17 min, isocratic for 13 min then to 100% over 5 min). Peaks 1, 2 and 3 were collected and purified by repeated HPLC. (B) Comparison of the UV spectra of oligonucleotide adduct peaks 1, 2 and 3 with the C8-dG-PhIP oligonucleotide adduct. Spectra were recorded in sodium phosphate (20mM, pH 7.0).