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Time-course images for living cells cultured in microfluidic device. The phase image of the MDCKs only (MDCK), MDCK cultured with collagen gel (MDCK+CG), or MDCKs cocultured with the ASCs encapsulating in collagen gel (MDCK+CG-ASC) were taken by 10×objective lens after seeding for 1, 24, and 48 h. The interface between MDCKs and collagen gel (yellow arrows) still remains clear after coculture for 48 h. Scale bar=200 μm.
