Table 1.
Histology Assessment, Number of Animals (%)
|
Surgical Procedure |
Cell Survival |
Monolayer* |
Subretinal Placement |
Pigment Migration |
Cell Reaction |
ONL Loss |
| hESC-RPE on membrane | ||||||
| 1 mo, n = 12 (%) | 12 (100) | 12 (100) | 8 (66.66) | 5 (41.66) | 11 (91.66) | 4 (33.33) |
| 6 mo, n = 12 (%) | 11 (91.66) | 9 (81.81) | 9 (75) | 8 (66.66) | 6 (50) | 11 (91.66) |
| 12 mo, n = 12 (%) | 6 (50) | 5 (83.33) | 9 (75) | 6 (50) | 7 (58.33) | 12 (100) |
| Total, n = 36 (%) | 29 (80.55) | 26 (89.65) | 26 (72.22) | 19 (52.77) | 24 (66.66) | 27 (75) |
| hESC-RPE suspension | ||||||
| 1 mo, n = 9 (%) | 7 (77.77) | 1 (14.28) | 6 (66.66) | 1 (11.11) | 3 (33.33) | 1 (11.11) |
| 6 mo, n = 12 (%) | 6 (50) | 1 (16.66) | 11 (91.66) | 5 (41.66) | 6 (50) | 10 (83.33) |
| 12 mo, n = 12 (%) | 3 (25) | 2 (66.66) | 11 (91.66) | 7 (58.33) | 7 (58.33) | 12 (100) |
| Total, n = 33 (%) | 16 (48.49) | 4 (22.22) | 28 (84.84) | 13 (39.39) | 16 (48.48) | 23 (69.69) |
hESC-RPE, human embryonic stem cell–derived retinal pigment epithelium.
*
Monolayer evaluation in the rats with cell survival (n = 45).