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Dot plots demonstrating separation of mixed fluorescence microspheres using PFCS. A BD Accuri flow cytometer was used to obtain the three plots where panel (a) is pre-sort Flow-Check fluorosphere and YG microsphere mixture, panel (b) and (c) display the number of separated YG and Flow-Check fluorospheres when PSFC was implemented. YG microspheres were purified to 90.64% and Flow-Check fluorospheres were purified to 91.70%.
