. Author manuscript; available in PMC: 2013 Jul 29.

Published in final edited form as: Anesthesiology. 2010 Sep;113(3):619–629. doi: 10.1097/ALN.0b013e3181e89ab2

Table 1.

BAL Cell Counts, Protein Content, IL-6 and MIP-2 Levels in VILI

	TLR4-WT			TLR4-mutant

	CON	LTV	HTV	CON	LTV	HTV
Protein content (μg/ml)	378.9 ± 71.4	386.0 ± 18.1	657.2 ± 25.4^*	333.6 ± 22.3	332.4 ± 59.3	475.7 ± 82.4^*, ^#
Cell counts (× 10⁴)	26.4 ± 2.2	27.5 ± 2.6	51.2 ± 1.1^*	17.3 ± 3.5	19.2 ± 3.4	28.3 ± 3.9^*, ^# (n=4)
IL-6 (BAL) (pg/ml)	133.4 ± 2.4	321.1 ± 103.9	452.5 ± 51.4^* (n=6)	92.6 ± 17.2	125.2 ± 3.9	265.7 ± 28.1^*, ^#
MIP-2 (BAL) (pg/ml)	16.5 ± 6.0 (n=6)	20.9 ± 1.8	68.3 ± 0.9^*	9.6 ± 3.3	17.7 ± 14.6	37.9 ± 3.7^*, ^# (n=4)
IL-6 (Plasma) (pg/ml)	30.6 ± 9.8	83.7 ± 13.8^†	110.5 ± 23.4^†	34.8 ± 13.6	71.1 ± 2.8^†	111.3 ± 39.4^*
MIP-2 (Plasma) (pg/ml)	1.5^§	1.6 ± 0.3	2.4 ± 0.9	1.5 ^§	2.2 ± 0.7 (n=4)	3.1 ± 1.5 (n=4)

Data are presented as mean ± SD.

P < 0.05 compared to strain-matched CON and LTV;

^†

P < 0.05 compared to strain-matched CON;

P < 0.05 compared to HTV in TLR4-WT mice. n = 5 in all groups except groups indicated in the table. Levels of MIP-2 in plasma were under detectable limits in unventilated control mice

^§

The detection threshold of the ELISA assay

BAL = bronchoalveolar lavage; CON = control; HTV = high tidal volume ventilation; IL-6 = interleukin 6; LTV = low tidal volume ventilation; MIP-2 = macrophage inflammatory protein 2; TLR4 = Toll-like receptor 4; VILI = ventilator-induced lung injury; WT = wildtype.