Table 1.
TLR4-WT | TLR4-mutant | |||||
---|---|---|---|---|---|---|
| ||||||
CON | LTV | HTV | CON | LTV | HTV | |
Protein content (μg/ml) | 378.9 ± 71.4 | 386.0 ± 18.1 | 657.2 ± 25.4* | 333.6 ± 22.3 | 332.4 ± 59.3 | 475.7 ± 82.4*, # |
Cell counts (× 104) | 26.4 ± 2.2 | 27.5 ± 2.6 | 51.2 ± 1.1* | 17.3 ± 3.5 | 19.2 ± 3.4 | 28.3 ± 3.9*, # (n=4) |
IL-6 (BAL) (pg/ml) | 133.4 ± 2.4 | 321.1 ± 103.9 | 452.5 ± 51.4* (n=6) | 92.6 ± 17.2 | 125.2 ± 3.9 | 265.7 ± 28.1*, # |
MIP-2 (BAL) (pg/ml) | 16.5 ± 6.0 (n=6) | 20.9 ± 1.8 | 68.3 ± 0.9* | 9.6 ± 3.3 | 17.7 ± 14.6 | 37.9 ± 3.7*, # (n=4) |
IL-6 (Plasma) (pg/ml) | 30.6 ± 9.8 | 83.7 ± 13.8† | 110.5 ± 23.4† | 34.8 ± 13.6 | 71.1 ± 2.8† | 111.3 ± 39.4* |
MIP-2 (Plasma) (pg/ml) | 1.5§ | 1.6 ± 0.3 | 2.4 ± 0.9 | 1.5 § | 2.2 ± 0.7 (n=4) | 3.1 ± 1.5 (n=4) |
Data are presented as mean ± SD.
P < 0.05 compared to strain-matched CON and LTV;
P < 0.05 compared to strain-matched CON;
P < 0.05 compared to HTV in TLR4-WT mice. n = 5 in all groups except groups indicated in the table. Levels of MIP-2 in plasma were under detectable limits in unventilated control mice
The detection threshold of the ELISA assay
BAL = bronchoalveolar lavage; CON = control; HTV = high tidal volume ventilation; IL-6 = interleukin 6; LTV = low tidal volume ventilation; MIP-2 = macrophage inflammatory protein 2; TLR4 = Toll-like receptor 4; VILI = ventilator-induced lung injury; WT = wildtype.