Figure 3.
Fluorescent micrographs of acridine orange (AO, green) and propidium iodide (PI, red) double-stained human SH-SY5Y cells viewed under fluorescence microscope. Bar = 20 μM; (a) Untreated control cells. (b) Treatment with 250 μM H2O2 for 1 h. (c) Cells pretreated with 1 ppm of GBR extract and subsequent treatment with 250 μM H2O2 for 1 h. (d) Cells pretreated with 10 ppm of GBR extract and subsequent treatment with 250 μM H2O2 for 1 h.