Figure 8.

Inhibition of GH6 CBHs by glucose. (A) ¹⁴C-BMCC (0.25 mg ml^-1) was incubated with 0.25 μM TrCel6A (opened symbols) or with 0.25 μM CtCel6A (filled symbols) 50°C. The concentration of added glucose was as follows: 0 M + 0.06 μM N188BG (◊,♦), 0.25 M (□, ■), 0.5 M (Δ, ▲), or 1.0 M (○, ●). (B) Hydrolysis data in coordinates (D_Glc/D_Glc=0) versus [glucose] where D_Glc and D_Glc=0 represent the degree of conversion of ¹⁴C-BMCC in the presence and absence of added glucose, respectively. (D_Glc/D_Glc=0) values for all hydrolysis time points are shown. Solid lines are from the non-linear regression according to Equation 5 (the terms referring to cellobiose were replaced with corresponding terms for glucose). CBH was TrCel6A (◊) or CtCel6A (×).