Figure 2. Effects of Nrf2 induction on ROS generation and extracellular H₂O₂ clearance.

Wild-type astrocytes were pretreated with vehicle or tBHQ. H₂O₂ was added as indicated and after 4 hours the generation of ROS was monitored by either A) DCFDA or B) DPPP. Astrocytes were pretreated with vehicle or tBHQ and the rate of H₂O₂ clearance from the extracellular medium was measured over time for C) Wild-type (WT) or D) Nrf2-knockout (Nrf2-KO) cells. Statistics were performed using 2-way ANOVA, * indicates p<0.01.