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. 2013 Jul 19;14:74. doi: 10.1186/1471-2350-14-74

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Copyright © 2013 Yan-Fang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The miR-663 promoter is methylated in pediatric AML. (A) MSP analysis of the methylation status of miR-663 shows aberrant methylation in pediatric AML samples compared to NBM/ITP control samples. M and U represent MSP results using primer sets for methylated and unmethylated miR-663 genes, respectively. (B) BGS results of three AML samples and three NBM samples show that CpGs are methylated in the AML samples compared to NBM control samples (91.3%, 84.8%, and 72.5% in AML10#, AML11#, and AML18#, respectively; 7.24%, 9.42%, and 9.42% in NBM1#, NBM2#, and NBM3#, respectively). ● methylated cytosines; ○ unmethylated cytosines.