Table 1.
Kinetic parameters for cellobiose hydrolysis by β-glucosidases
| |
kcat(h) (s-1)a |
KM(h)(mM)a |
||||
|---|---|---|---|---|---|---|
| t (°C) | N188BG | TaBG3 | AtBG3 | N188BG | TaBG3 | AtBG3 |
| 25 |
121 ± 4 (70%) |
227 ± 10 (57%) |
105 ± 11 (95%) |
0.73 ± 0.04 |
0.42 ± 0.03 |
0.29 ± 0.07 |
| 35 |
271 ± 5 (72%) |
401 ± 19 (57%) |
180 ± 11 (92%) |
0.97 ± 0.04 |
0.51 ± 0.04 |
0.32 ± 0.04 |
| 45 |
493 ± 12 (70%) |
632 ± 20 (61%) |
326 ± 14 (81%) |
1.34 ± 0.06 |
0.60 ± 0.03 |
0.41 ± 0.04 |
| 55 |
691 ± 27 (76%) |
1058 ± 81 (60%) |
666 ± 23 (66%) |
1.36 ± 0.12 |
0.67 ± 0.10 |
0.87 ± 0.05 |
| 65 | 1497 ± 53 (67%) | 968 ± 31 (71%) | 0.82 ± 0.06 | 0.93 ± 0.06 | ||
The values in parentheses show the highest measured value of the rate constant for cellobiose hydrolysis as a percentage of kcat(h).
aThe values of kcat(h) and KM(h) were determined by a non-linear regression analysis of the data of cellobiose turnover, according to Equation 2.