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. 2013 Jul 24;6:105. doi: 10.1186/1754-6834-6-105

Table 5.

Kinetic parameters of selected β-glucosidases

Organism
  
  
 kcat (s-1)
 KM (mM)
 kcat/KM (105 M-1 s-1)
 Ki glucose (mM)
 Ki/KMa
 Ref
  t°C pH CB pNPG CB pNPG CB pNPG on CB on pNPG    
Penicillium verruculosum
  
  
 118b
 650b
 0.36
 1.6
 3.29
 4.06
  
 0.19
 0.53
 [34]
Phanerochaete chrysosporium
 22
 4
 50
 132
 2.3
 0.10
 0.22
 13.8
  
 0.27
 0.12
 [35]
Myceliophthora thermophila
 40
 5
 46
 147
 2.64
 0.39
 0.17
 3.76
  
 0.28
 0.11
 [36]
Thermoascus aurantiacus
 60
 4.5
 284
 242
 0.64
 0.11
 4.46
 21.2
  
 0.29
 0.45
 [37]
Trichoderma reesei
 50
 4.5
 22
  
 0.54
  
 0.41
  
 0.29
  
 0.54
 [38]
Fomitopsis palustris
 50
 5
 102
 721
 4.8
 0.12
 0.21
 61.6
  
 0.35
 0.07
 [39]
Acremonium thermophilum
 55
 5
 666
  
 0.87
  
 7.65
  
  
 0.50
 0.57
 Tc
Magnaporthe grisea
 50
 5
  
  
 1.1
  
  
  
 0.5
  
 0.45
 [8]
Trichoderma reesei
 40
 5
 42
 118
 0.75
 0.09
 0.56
 13.1
  
 0.51
 0.68
 [40]
Chaetomium globosum
 50
 5
 168
  
 0.95
  
 1.77
  
 0.68
  
 0.72
 [21]
Trichoderma reesei
 40
 5
 29
 70.8
 1.25
 0.1
 0.23
 6.94
  
 0.7
 0.56
 [41]
Penicillium verruculosum
 40
 5
 89
 160
 1.2
 0.44
 0.74
 3.64
  
 0.93
 0.78
 [40]
Aspergillus fumigatus
 50
 5
 768
  
 1.77
  
 4.34
 0.00
 1.1
  
 0.62
 [21]
Penicillium brasilianum
 22
 4.8
 53.7b
 146b
 1.58
 0.09
 0.34
 16.2
 1.1
 2.3
 0.70
 [20]
Thermoascus aurantiacus
 55
 5
 1058
  
 0.67
  
 15.5
  
  
 1.17
 1.75
 Tc
Aspergillus niger (N188)
 22
 4.8
  
  
 0.35
 0.45
  
  
 1.6
 1.1
 4.57
 [20]
Emericella nidulans
 50
 5
 87
  
 2.32
  
 0.38
  
 1.83
  
 0.79
 [21]
Aspergillus niger (N188)
 50
 5
 558
  
 1.15
  
 4.85
  
 1.94
  
 1.69
 [21]
Fusarium oxysporum
 50
 5
 323
 7.7
 1.07
 0.09
 3.02
 0.83
  
 2.05
 1.92
 [42]
Penicillium brasilianum
 50
 5
 520
  
 2.05
  
 2.54
  
 2.3
  
 1.12
 [21]
Aspergillus japonicus
 40
 5
 350
 259
 0.95
 0.6
 3.68
 4.32
  
 2.73
 2.87
 [40]
Aspergillus niger
 25
 4.5
 104b
 61b
 2.7
 1
 0.38
 0.61
  
 3
 1.11
 [43]
Aspergillus niger (N188)
 55
 5
 691
  
 1.36
  
 5.07
  
  
 3.12
 2.29
 Tc
Trichoderma reesei
 50
 4.8
 41
 87.9
 1.36
 0.38
 0.30
 2.31
  
 3.25
 2.39
 [23]
Aspergillus oryzae
 50
 5
 363
  
 1.78
  
 2.04
  
 3.26
  
 1.83
 [21]
Aspergillus niger (N188)
 50
 4.8
 32
 23.4
 0.88
 0.57
 0.36
 0.41
 3.4
 2.7
 3.86
 [23]
Aspergillus oryzae
 50
 5
 1000
 370
 1.96
 0.29
 5.10
 12.7
 5
 2.9
 2.55
 [22]
Aspergillus niger
 40
 4
 2780
 917
 15.4
 2.2
 1.81
 4.17
  
 5.7
 0.37
 [44]
Aspergillus tubingensis
 30
 4.6
 331b
 140b
 1
 0.76
 3.31
 1.83
  
 5.8
 5.80
 [45]
Penicillium italicum
 60
 4.5
 2641
 1746
 0.41
 0.11
 64.4
 158
  
 8.9
 21.7
 [25]
Aspergillus japonicus
 30
 5
 46b
 54.5b
 1.16
 0.2
 0.40
 2.72
  
 9.2
 7.93
 [46]
Neurospora crassa
 50
 5
 423
 640
 2.95
 2.54
 1.43
 2.52
 10.1
 6.43
 3.42
 [21]
Aspergillus sp
 60
 4.5
  
  
  
 1.0
  
  
  
 17
 17
 [47]
Periconia sp
 40
 5
 972b
 1180b
 0.5
 0.19
 19.4
 62.7
  
 20
 40.0
 [48]
Baltic sea metagenome
 30
 6.5
 11.2
 22.5
 2.76
 0.37
 0.04
 0.61
  
 30
 10.8
 [49]
Aspergillus niger (N188)
 45
 5
  
  
 16.8
 1.77
  
  
 59.5
 1.59
 3.54
 [24]
Streptomyces sp
 50
 6.5
 35.6
 28.4
 4.1
 0.15
 0.09
 1.89
  
 65
 15.8
 [50,51]
Torulopsis wickerhamii
  
  
  
 362b
 300
 2.8
  
 1.29
  
 190
 0.63
 [52]
Thermoascus aurantiacus
 40
 5
 0.72b
 5.08b
  
 0.2
  
 0.25
  
 300
  
 [27]
Pyrococcus furiosus
 95
 5
 454
 677b
 20
 0.15
 0.23
 45.1
  
 300
 15.0
 [53]
Debaromyces vanrijiae
 40
 5
 141b
 1113b
 57.9
 0.77
 0.02
 14.5
  
 439
 7.58
 [54]
Aspergillus niger
 40
 4
 4.3b
 223
  
 21.7
  
 0.10
  
 543
  
 [28]
Thermoanaer. thermosacch.
 70
 6.4
 104b
 55b
 7.9
 0.63
 0.13
 0.88
  
 600
 75.9
 [29]
Uncultured bacterium
 40
 6.5
 13.2b
 43b
 20.4
 0.39
 0.01
 1.11
  
 1000
 49.0
 [55]
Aspergillus oryzae
 50
 5
 253b
 764b
 7
 0.55
 0.36
 13.9
  
 1360
 194
 [30]
Candida peltata 50 5 54b 158b 66 2.3 0.01 0.69   1400 21.2 [31]

BGs are listed in the order of increasing Ki(Glc). If Ki(Glc) values measured using both pNPG and cellobiose (CB) as the substrate were available, the priority was given to the Ki(Glc) value measured using cellobiose.

aKM is for cellobiose hydrolysis. If Ki(Glc) values measured using both pNPG and cellobiose (CB) as the substrate were available, the priority was given to the Ki(Glc) value measured using cellobiose.

bCalculated from the reported specific activity and molecular weight of the enzyme.

cThis study.