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. 2001 May 1;29(9):1844–1851. doi: 10.1093/nar/29.9.1844

Figure 3.

Figure 3

Molecular characterisation of the LdTOP2 locus. (A) Southern blot showing that the LdTOP2 is a single copy gene. Lanes 1–5, restriction digests of L.donovani D1700 genomic DNA with SalI, HindIII, PstI, EcoRV and EcoRV–XbaI, respectively, and their hybridisations with the 800 bp gene internal probe of LdTOP2 corresponding to positions 1537–2335. The positions of DNA molecular weight markers are indicated on the left of the figure. (B) Chromosomal location of the LdTOP2 gene. a, Chromosomes of S.cerevisiae and L.donovani D1700 separated and visualised with ethidium bromide before blotting; b, the same blots probed with yeast genomic DNA for S.cerevisiae and LdTOP2 gene probe for L.donovani. Arrow shows the hybridising band of L.donovani. PFGE running conditions as follows: initial switch time, 1800 s; final switch time, 1800 s; run time, 72 h; current, 2 V/cm; including angle, 106°; actual current, 30 mAmp.