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. 2013 Jul 29;8(7):e70221. doi: 10.1371/journal.pone.0070221

Figure 3. Marker imposition and watershed segmentation for nuclei segmentation.

Figure 3

Prior to applying the FRST the image is preprocessed with color unmixing and morphological operations (n = 10). The set of radii for the FRST is R = (10, 11,…,20). Note: the markers and watershed ridges (given in green in the figure) were dilated by one pixel for better visualization. A) Original image. B) Hematoxylin channel. C) Pre-processed image (hematoxylin channel processed with series of morphological operations). D) Fast radial symmetry transform (FRST). E) FRST foreground and background markers. F) Watershed segmentation with FRST markers. G) Regional minima foreground and background markers. H) Watershed segmentation with regional minima markers.