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. 2013 Jul 16;13:32. doi: 10.1186/1472-6831-13-32

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Copyright © 2013 Dorkhan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Images showing biofilm formation by S. oralis on uncoated titanium surfaces. Bacteria suspended in PBS were allowed to form biofilms on titanium surfaces in a parallel plate flow-cell system for 2 and 24 hours. The viability of the cells was assessed by Live/Dead BacLight staining and viewing with CSLM. Surface coverage on the titanium plates was assessed from ten random images using the bioImage_L software package. The scale bars represent 10 μm and the inserts show cells in twofold enlargement.