Figure 5.

Seasonal enrichment analysis of H2A.Z on carp ribosomal cistron. (A) Schematic representation of genomic organization of the carp ribosomal cistron. Green stars indicate the regions of the qPCR amplicons associated in ChIP experiments. (B) The seasonal enrichment of H2A.Z in rDNA cistron is shown. (C) The seasonal enrichment of H2A.Z on RNA polymerase II promoters (L41, Δ⁹-desaturase, and JAK1) is shown (n = 3 in each season). The data presents real-time PCR measurements of the immunoprecipitated DNA at the corresponding rDNA regions. Standard deviations (± SD) are shown (*P <0.001, **P <0.005, Student’s t-test). (D) Seasonal enrichment of canonical histone H3 in rDNA cistron. ChIP, chromatin immunoprecipitation; qPCR, quantitative PCR; SD, standard deviation.