Figure 5.
(A and B) Increased 868 TCR-transduced CTL cytotoxicity at 40°C compared with 37°C. (A) Fluorometric assessment of T-lymphocyte antigen-specific lysis killing assays were performed in duplicate and analyzed over time using SLYNTVATL pulsed T2 cells and 868 TCR CTLs at 1:1 or 1:2 effector:target ratios at different temperatures (37°C or 40°C). Decreases in the peptide-pulsed carboxyfluorescein succinimidyl ester-positive cell population (fluorescein isothiocyanate) were measured, as a percentage of the control-pulsed cell population, where no CTLs were added (percentage cytotoxicity as a percentage of control). (B) Percentage cytotoxicity (as a percentage of control) using 10−7 M SLYNTVATL-pulsed T2 cells was determined after a 5-hour incubation as above, with eight replicates at 37°C or 40°C.
Abbreviations: TCR, T-cell receptor; CTL, cytotoxic T lymphocyte.