Figure 4. PIN6 overexpression affects organ development and waving in roots.

A). Root architecture of wild type and PIN6-OE (#33 and #1) seedlings (10 DAG). B) Close up of PIN6-OE#1 waving roots. C) PIN6-OE lines show a reduction in root elongation (10 DAG). D) Root biomass of plants growing in soil was quantified 4 weeks after germination. Average±SE (n = 12). E) and F) Wave amplitude and wave length, respectively, for primary roots. Error bars show standard error (n = 9 to 133) from a single experiment representative of three independent experiments. G) Root hair outgrowth (4 DAG) is disrupted by PIN6 overexpression, when compared to wild type. H) Confocal micrographs show the development of trichoblast cells (PIN6-OE#1) or emergence of root hairs in WT. White arrows indicate root hair cells and PIN6-OE#1 is representative of multiple lines examined. I) Cross section of the primary root from wild type and PIN6-OE#14, respectively showing the position of a H (hair) cell in between to two N (no-hair) cells. Similar results were obtained for PIN6-OE#1. J). Development stage distribution of lateral root primordia initiated (I-VI) as well as emerged (Em) (9 DAG; n = 15) representative of multiple experiements. K) Primordium density of lateral root initiation (In) and emergence (Em) per cm of root length (8 DAG; n = 20). O) and P) Cellular patterning of root primordium at different developmental stages from initiation to emergence in PIN6 OE#1 and wild type (WT). All scale bars represent 10 µm and images are representative of multiple primordia examined. When necessary homogeneity groups according to Student’s t test (p<0.05) are denoted with same letters. Error bars represent standard error.