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. 2013 Jul 29;8(7):e70277. doi: 10.1371/journal.pone.0070277

Figure 8. Modulators of DAG pathway affect the SSE and the persistent PTD differently.

Figure 8

A, Application of 1 µM PDBu increases fEPSP/FV (top) and decreases PPF (middle) consistent with an increase in release probability. This increase in release probability does not appear to be mediated by an increase in presynaptic calcium signaling as indicated by the lack of effect of PDBu on ΔF/F signals arising from GCamP2 in PFs (bottom). B-C, fEPSP/FV is plotted. Vertical dashed grey line indicates stimulation with a 10-pulse burst at 200 Hz. In 1 µM PDBu, the SSE is reduced and the persistent PTD is abolished (n = 10) (B). When PDBu is applied in the presence of 2 µM Gö6983 (pre-incubated for 1 hour), a broad spectrum PKC blocker, the SSE is potentiated and the persistent PTD expression is rescued (n = 5) (C). Gö6983 alone has similar effects (n = 10) (C). D, Average effects of 1 µM PDBu, 1 µM PDBu +2 µM Gö6983 or 2 µM Gö6983 on the SSE or the persistent PTD amplitude. One or two asterisks are for p = 0.02 or p<0.01, respectively, relative to control conditions.