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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Membr Biol. 2013 Mar 24;246(10):737–744. doi: 10.1007/s00232-013-9534-y

Figure 5. SMGA functions in vivo as a nuclear targeting sequence.

Figure 5

Rat mesenteric arteries were electroporated with pCMV-GFP-SMGA or no DNA and 2 days later harvested. GFP expression could be detected directly in whole vessels. Cryosections were immunostained with anti-GFP (green) or anti-SMαA (red) and reacted with DAPI. Paraffin sections were immunostained with anti-GFP antibodies and visualized with VectaRed or stained with H&E. In both types of thin sections (vessels from different animals), GFP expression was restricted to the smooth muscle layer (Young et al., 2008).