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. 2013 Jul 29;8(7):e70988. doi: 10.1371/journal.pone.0070988

Figure 5. Reversal of the effect of Klotho on electrogenic glutamate transport in EAAT3 or EAAT4 expressing Xenopus oocytes by β-glucuronidase inhibitor DSAL.

Figure 5

A: Means ± SEM (n = 9–21) of glutamate (2 mM)-induced current (Iglu) in Xenopus oocytes injected with water (grey bar) or injected with cRNA encoding EAAT3 alone (white bar) or both EAAT3 and Klotho (black bars). Where indicated, the oocytes were treated with β-glucuronidase inhibitor DSAL (10 µM).**(p<0.01) indicates statistically significant difference from oocytes injected with cRNA encoding EAAT3 alone (ANOVA). ### (p<0.001) indicates statistically significant difference from oocytes injected with cRNA encoding both EAAT3 and Klotho (ANOVA).B: Means ± SEM (n = 11–23) of glutamate (2 mM)-induced current (Iglu) in Xenopus oocytes injected with water (grey bar) or injected with cRNA encoding EAAT3 alone (white bar) or pretreated for 24 hours with 30ng/ ml recombinant human β-Klotho protein without (first black bar) or with the presence of β-glucuronidase inhibitor DSAL (10µM ) (second black bar).***(p<0.001) indicates statistically significant difference from non-treated oocytes injected with cRNA encoding EAAT3 alone (ANOVA). ##(p<0.01). indicates statistically significant difference from oocytes injected with cRNA encoding EAAT3 and treated for 24 hours with 30ng/ ml recombinant human β-Klotho protein (ANOVA).C: Means ± SEM (n = 5–23) of glutamate (2 mM)-induced current (Iglu) in Xenopus oocytes injected with water (grey bar) or injected with cRNA encoding EAAT4 alone (white bar) or both EAAT4 and Klotho (black bars). Where indicated, the oocytes were treated with β-glucuronidase inhibitor DSAL (10 µM).***(p<0.001) indicates statistically significant difference from oocytes injected with cRNA encoding EAAT4 alone (ANOVA). ###(p<0.001) indicates statistically significant difference from oocytes injected with cRNA encoding both EAAT4 and Klotho (ANOVA).D: Means ± SEM (n = 9–21) of glutamate (2 mM)-induced current (Iglu) in Xenopus oocytes injected with water (grey bar) or injected with cRNA encoding EAAT4 alone (white bar) and pretreated for 24 hours with 30 ng/ ml recombinant human β-Klotho protein without (first black bar) or with the presence of β-glucuronidase inhibitor DSAL (10µM, second black bar).***(p<0.001) indicates statistically significant difference from non-treated oocytes injected with cRNA encoding EAAT4 alone (ANOVA). ##(p<0.01). indicates statistically significant difference from oocytes injected with cRNA encoding EAAT4 and treated for 24 hours with 30 ng/ml recombinant human Klotho protein (ANOVA).