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. 2013 Jul 29;8(7):e70428. doi: 10.1371/journal.pone.0070428

Figure 4. Imetelstat increases FLU-induced γH2AX and decreases FLU-induced pDNA-PK.

Figure 4

A) The graph represents the mean levels of γH2AX (phosphorylated H2AX) (y-axis) after treatment of CLL lymphocytes as indicated (x-axis) in eight samples. The whiskers represent the standard error for each group of four samples tested. One Way Analysis of Variance, *p<0.001. B) The median sensitization R values (x-axis) in CLL lymphocyte samples where Imetelstat did not affect FLU-induced γ-H2AX (left bar) was significantly lower than in CLL lymphocyte samples where Imetelstat increased FLU-induced γH2AX (right bar) with respective median values of 1.4 and 1.8 (t-test *p<0.05). C) Changes in DNA-PK serine 2056 (pDNA-PK) in ten primary CLL lymphocyte samples treated with FLU alone (IC50) or in combination with 1 µM Imetelstat. The values in the y-axis represent fold change in pDNA-PK with respect to vehicle treated lymphocytes. 1 µM M Imetelstat significantly decreased FLU-induced pDNA-PK (Wilcoxon Signed Rank Test, p = 0.004).