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. 2013 Jul 29;8(7):e70334. doi: 10.1371/journal.pone.0070334

Figure 4. QYHJ suppresses cancer-related inflammation in pancreatic cancer.

Figure 4

A. An orthotopic nude mouse model was established and treated with QYHJ or vehicle as described above. Blood was collected from each mouse, and the serum concentrations of the pro-inflammatory cytokines IL-6 and TNF-α were detected by ELISA. The Student’s t-test was used to determine the statistical significance. B. To evaluate TAM infiltration, CD68 staining was performed using IHC. Original magnification, 200×. TAM infiltration was evaluated quantitatively by calculating the ratio of the CD68-positive area to the total area in each field, and the mean value from ten fields under 200× microscopy was determined. *P<0.05. C. Confocal microscopy of pancreatic cancer tissues treated with anti-CD68 (red) and anti-IL-6 (green). Cell nuclei were counterstained with DAPI.