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. 2013 Jul 29;8(7):e70334. doi: 10.1371/journal.pone.0070334

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© 2013 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A. Cell migration rates of pancreatic cancer cells treated with IL-6 (100 ng/mL) or vehicle only were compared via wound healing assays. Microscopic observations were recorded at 48 hours after scratching the surface of a confluent layer of cells. Asterisk indicates the wound was completely healed. B. The invasive capacity of pancreatic cancer cells treated with IL-6 (100 ng/mL) or vehicle only were compared using Matrigel invasion assays. The numbers of cells that traveled through the membrane were counted in 10 fields under a ×20 objective lens. Original magnification, ×200. The results represent the means ± SD of values obtained in three independent experiments. Statistical significance was calculated using the Student’s t-test.