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. 2013 Jul 29;8(7):e70652. doi: 10.1371/journal.pone.0070652

Figure 3. Focal Adhesion Maturation under Treatment with ROCK inhibitor Y-27632.

Figure 3

(a) Time-lapse images of GFP-actin and mApple-paxillin in U2OS cell treated with 2 µM Y-27632, with images of traction stress magnitude. Time is in min:sec. (b) Focal adhesion length (black squares) and traction stress (blue circles) at the focal adhesion indicated in (a) as a function of time. Focal adhesion assembly characteristics in varied Y-27632 concentrations: (c) FA elongation rate, (d) FA loading rate, (e) FA disassembly rate, (f) FA lifetime under indicated conditions (n = 10 FA for each condition for FA elongation, loading rate, disassembly rate, and lifetime). (g) Lamellar actin retrograde flow speeds in varied conditions (n = 2 cells per condition, 5 to 7 regions per cell). (h) Box plot of peak traction stress reached during focal adhesion lifetimes in cells treated with Y-27632. “Stable FA” indicates FA that had lifetime greater than 30 minutes, while “Unstable FA” indicates FA with lifetime less than 30 minutes.