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. 2013 Jul 29;8(7):e69422. doi: 10.1371/journal.pone.0069422

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© 2013 Dacks et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Western blots of the AM3-A antibody against Manduca protein and in which the AM3-A antibody was omitted (no 1^o) produced no labeling. (B) Frontal section through an AL labeled using an antibody against a 5-HT_1A receptor from prawn (AM3-A) generously provided by Dr. Maria Sosa. Note cell bodies (arrowheads; 24±2) and fine processes (arrows). (C) Manduca ALs labeled with AM3-A antibody pre-adsorbed with the Ms5HT1A receptor sequence (KDPDYLARITQQQKCLVSQD) homologous to the antigenic sequence used to generate the AM3-A antibody (KDPDFLVRVNEHKKCLVSQD) resulted in no labeling. (D) Double labeling against the Ms5HT1A receptor (green) and 5-HT (magenta) show no overlap. (E) Backfills of projection neurons (PNs) (magenta) reveal no co-localization of the Ms5HT1A receptor (green) in the lateral cell cluster of the AL. Magenta arrows indicate PNs and green arrows indicate Ms5HT1A-ir neurons. (F) Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). All scale bars=100µm.