Table 3. The effect of synthetic Antagonists on the Ms5HT1A and Ms5HT1B receptors.
| Drug | Receptor Subtype Specificity | 5HT1A receptor | n | 5HT1B receptor | n |
|---|---|---|---|---|---|
| 5HT | Non-specific | 100% (150.5 ± 14.2 nA) | 14 | 100% (100.5 ± 17.6 nA) | 30 |
| WAY100635 | 5HT1A | 76.8 ± 15.8% | 3 | 38.2 ± 3.3% | 7 |
| Methiothepin | 5HT1,5HT6,5HT7 | 111.7 ± 2.7% | 3 | 36.4 ± 12.6% | 7 |
| Spiperone | 5HT2A | 116.3 ± 15.7% | 5 | 82.4 ± 16.5% | 4 |
| Mianserin | Non-specific | 112.3 ± 3.5% | 3 | 103.0 ± 3.6% | 3 |
| Prazosin | Non-specific | 124.1 ± 14.2% | 8 | 93.0 ± 18.9% | 8 |
The 5HT1A responses were measured in oocytes coinjected with receptor cRNA and cRNA for the CFTR channel while the 5HT1B responses were measured in oocytes injected with the receptor cRNA alone. Oocytes were exposed to a 2 min pulse of 1 µM 5-HT in the presence of antagonists at a concentration of 3 µM. Oocytes were preincubated for 5 min in antagonist before 5-HT application. The results are expressed as a percentage of the response to a control pulse of 1 µM 5-HT in the same oocytes in the absence of antagonists.