Table 4. Effects of Methysergide on the Ms5HT1A, 2 and 7 receptors.
| Drug | Ms5HT1A | n | Ms5HT2 | n | Ms5HT7 | n |
|---|---|---|---|---|---|---|
| Methysergide alone | 27.8 ± 8% | 6 | 0% | 6 | 49.5 ± 12% | 6 |
| 5-HT alone | 100% (137.0 ± 6.5nA) | 6 | 100% (382.3 ± 37.9nA) | 6 | 100% (328.0 ± 14.7 nA) | 6 |
| Methysergide + 5-HT | 101.0 ± 27% | 6 | 110.1 ± 24% | 6 | 87.8 ± 16% | 6 |
The 5HT1A responses were measured in oocytes coinjected with receptor cRNA and cRNA for the CFTR channel while the 5HT2 and 5HT7 responses were measured in oocytes injected with the receptor cRNA alone. Oocytes were exposed to 2 minute pulses of either 3µM methysergide, 1µM 5-HT or 3µM methysergide and 1µM 5-HT. Results are expressed as a percentage ± SEM of the response of the same oocytes to 2 min control pulses of 5HT.