Figure 8. Treatment with adult barnacle crude extract significantly activated MKK3 and p38MAPK.

Freshly transformed cyprids were treated with 20 or 40 µg/ml adult barnacle extract for 10 hours. The same concentration of BSA was used as protein control and cyprids without treatment were used as blank control. The relative levels of pp38MAPK and pMKK3 were determined by Western blot. Phosphorylation levels of MKK3 and p38MAPK in blank control were assigned unit of 1 and used for normalization. Multiple comparison analysis showed that both 20 and 40 µg/ml of adult barnacle extract significantly induced the activation of p38MAPK and MKK3, but the same concentration of BSA did not have any effects. The asterisks indicate significant differences from the blank control.