Fig 3.

ICG-001 modulates WNT/catenin activity and apoptosis in p300 deficient HCT-R CRC cells. (A) Western blot data confirming expression of CBP and lack of p300 expression in HCT-R cells, mock treated (M) or treated with 5 mM butyrate (B). In the p300 immunoblot, non-specific background bands are present, but p300 expression is not observed. (B) ICG-001 inhibits butyrate-induced WNT/catenin activity in HCT-R cells. Experiments were performed as described in ref. 29. Data are from three separate experiments. Bars, SDs. * = statistical significance. (C) Butyrate interferes with upregulation of apoptosis by ICG-001 in HCT-R cells. Experiment performed as in the Materials and Methods section. Data are from four separate experiments. Bars, SDs. * = statistical significance.