Table 3. De novo assemblies of reads generated for archival plant, fungal and insect specimens, and fresh control tissues.
| Specimen, type of material | Velvet settings1 | Assembly size (Mb) | N50 (nt) | Contigs | Alignable contigs2 | % Genome coverage3 | Reference genome |
| Plant herbarium specimen | |||||||
| Arabidopsis thaliana, herbarium | 27* | 67.1 | 1,107 | 65,388 | 64,024 | 55.5 (16.2) | A. thaliana nuclear, GCF_000001735.3 |
| Arabidopsis thaliana, herbarium | 47, 50, 150* | 0.19 | 15,211 | 34 | 8 | 98.4 | A. thaliana chloroplast, NC_000932 |
| Labunum anagyroides, herbarium | 47, 50, 2000 | 0.82 | 3,614 | 280 | 3 | 81.2 | G. max chloroplast, NC007942 |
| Liriodendron tulipifera, herbarium | 39, 150* | 0.18 | 22,290 | 34 | 6 | 100 | L. tulipifera chloroplast, NC_008326 |
| Plant fresh tissue | |||||||
| Arabidopsis thaliana, fresh tissue | 27* | 78.0 | 1,154 | 73,838 | 65,612 | 60.9 (19.8) | A. thaliana nuclear, GCF_000001735.3 |
| Arabidopsis thaliana, fresh tissue | 47, 50, 150* | 0.14 | 9,114 | 28 | 18 | 97.2 | A. thaliana chloroplast, NC_000932 |
| Laburnum anagyroides, fresh tissue | 47, 50, 2000 | 1.49 | 12,726 | 223 | 3 | 81.4 | G. max chloroplast, NC007942 |
| Liriodendron tulipifera, fresh tissue | 39, 150* | 0.21 | 8,296 | 43 | 15 | 99.2 | L. tulipifera chloroplast, NC_008326 |
| Fungal herbarium specimen | |||||||
| Agaricus bisporus, herbarium | 41, 5, 50 | 27.7 | 20,217 | 1,820 | 1,720 | 81.1 (71.0) | A. bisporus H97 v2.0 nuclear, MycoCosm |
| Agaricus bisporus, herbarium | 41, 50, 2000 | 0.24 | 41,776 | 43 | 2 | 99.9 | A. bisporus H97 v2.0 mitochondrion, MycoCosm |
| Laccaria bicolor, herbarium | 41, 5, 50 | 38.8 | 19,276 | 2,559 | 2,495 | 62.0 (34.4) | L. bicolor v2.0 nuclear, MycoCosm |
| Laccaria bicolor, herbarium | 51, 50, 1000 | 1.55 | 6,809 | 384 | 5 | 24.5 | C. cinerea okayama7#130 mitochondrion, Broad |
| Pleurotus ostreatus, herbarium | 41, 5, 100 | 34.3 | 85,861 | 858 | 725 | 78.5 (56.4) | P. ostreatus PC15 v2.0 nuclear, MycoCosm |
| Pleurotus ostreatus, herbarium | 41, 50, 300 | 2.24 | 9,705 | 405 | 9 | 90.2 | P. ostreatus mitochondrion, EF204913 |
| Archived insect specimen | |||||||
| Aedes albopictus, archived4 | xx | xx | Xx | xx | xx | xx | |
| Anoplophora glabripennis, archived | 51, 20* | 0.15 | 2,022 | 77 | 0 | 0 | A. glabripennis mitochondrion, NC_008221 |
| Ceratitis capitata, archived (leg) | 39, 150* | 0.04 | 5,249 | 12 | 1 | 96.8 | C. capitata mitochondrion, NC_000857 |
| Ceratitis capitata, archived (head/thorax) | 39, 150* | 0.03 | 3,282 | 11 | 2 | 96.8 | C. capitata mitochondrion, NC_000857 |
| Insect fresh tissue | |||||||
| Anoplophora glabripennis, fresh | 51, 20* | 0.43 | 3,650 | 106 | 5 | 90.3 | A. glabripennis mitochondrion, NC_008221 |
| Ceratitis capitata, fresh (leg) | 39, 150* | 0.05 | 3,794 | 17 | 2 | 94.2 | C. capitata mitochondrion, NC_000857 |
| Ceratitis capitata, fresh (head/thorax) | 39, 150* | 0.05 | 2,778 | 25 | 2 | 96.5 | C. capitata mitochondrion, NC_000857 |
1 Velvet setting: k-mer length, coverage cutoff and expected coverage. * Analysis was run in single-end modus.
2 The number reference-sequence alignable contigs.
3 Percentage coverage of coding sequences (CDS) is given in brackets.