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. 2013 Jul 29;8(7):e69846. doi: 10.1371/journal.pone.0069846

Figure 7. Generation of Th1/Th17 mediated immunity in response to C. difficile in an in-vitro murine model system.

Figure 7

WT BMDCs were infected with PFA-fixed C. difficile strains. 24 h post-infection, stimulated BMDCs were co-cultured with WT splenocytes in the presence of anti-CD3/CD28. 96 h post co-culture, secreted cytokines IFN-γ (A), IL-17A (B), IL-10 (C), IL-4 (D) and IL-2 (E) were measured by ELISA. 1 µg/ml LPS stimulation served as reference control. Data represent mean ± SEM, n = 3. */∧p<0.05, **/∧∧p<0.01 and ***p<0.001 represent significant difference when compared to uninfected control cells and significant inter-strain difference, respectively. P values were obtained using ANOVA with Bonferroni post-test analysis.