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. 2013 Jul 29;8(7):e69790. doi: 10.1371/journal.pone.0069790

Figure 2. Purification and CD spectral analysis of rRv2599.

Figure 2

A. Protein topology showing the presence of signal peptide and/or transmembrane helices in the protein signal peptide spans from 1–16 amino acids, whereas transmembrane domain spans from 7–29 amino acids. B. Purified protein run on 12% SDS-PAGE stained with coomassie. Lane M is molecular weight marker and Lane 1 is the purified recombinant rRv2599. C. Western blotting with Anti-His antibodies. Lane M is molecular weight marker and Lane 1 is the purified recombinant rRv2599. D. CD spectra of purified rRv2599 at two different temperatures 25°C and 37°C.