Table 1. List of the primers used in the present study.

ORF	Initial amino acids removed	Orientation	Primers	Restriction Enzyme
Rv0309	29	Forward Primer	5′ – AGC CTG GCC GGA TCC AAC CCG TGG TTC GCG – 3′	BamHI
		Reverse Primer	5′ –CGT CGC ACA GTT CTC GAG CGA GGT CCG GGG – 3′	XhoI
Rv2599	29	Forward Primer	5′ – ATC ACG CTG GGA TCC AGG GAC GTT GGC TCG – 3′	BamHI
		Reverse Primer	5′ –ATA CAT GAC TCT CTC GAG TGC GTC ATC GCC – 3′	XhoI
Rv3717	24	Forward Primer	5′-ACC CCC GCC GGA TCC GCC GGC ATG GTC GTC-3′	BamHI
		Reverse Primer	5′-GCG CGG TGG GGG CTC GAG CTG TGT GCG GGG -3′	XhoI