Table 2. List of proteins meeting selection criteria, with high SPAAN score, globular proteins and have no reference in literature.
| RvID | Protein* | Size (kDa) | SPAAN (Pad Value) | Localization | Globular Proteins | CDD Domain Analysis (E Value)$ |
| Rv1116A | Conserved hypothetical protein | 9.040 | 0.717 | Extracellular | Globular | None |
| Rv1987 | Possible chitinase | 14.920 | 0.670 | Extracellular | Globular | Carbohydrate binding domain, Cellulose binding domain (4.69e-24) |
| Rv1881c | lppE (Lipoprotein) | 14.940 | 0.664 | Extracellular | Globular | Myco_19_kDa super family (6.82e-04) |
| Rv2599 | Probable conserved membrane protein | 14.950 | 0.752 | Extracellular | Globular | Domain of unknown function (DUF4247) (2.07e-31) |
| Rv1984c | cfp21 (Hydrolyzes cutin) | 21.780 | 0.685 | Extracellular | Globular | Cutinase (2.20e-52) |
| Rv0309 | Conserved exported protein | 22.530 | 0.691 | Extracellular | Globular | L,D-transpeptidase catalytic domain, YkuD, COG3786 (1.43e-79) |
| Rv3576 | lppH (Possible conserved lipoprotein) | 25.020 | 0.693 | Extracellular | Globular | PknH-like extracellular domain (3.65e-47) |
| Rv3822 | Conserved hypothetical protein | 41.410 | 0.739 | Extracellular | Globular | PE-PPE domain (1.53e-68) |
| Rv0050 | ponA (Peptidoglycan synthesis) | 71.120 | 0.688 | Extracellular | Globular | Penicillin binding protein transpeptidase domain (1.41e-49) |
| Rv0584 | Possible conserved exported protein | 92.950 | 0.744 | Extracellular | Globular | Glycosyl hydrolase family 92 (0e+00) |
| Rv1037c | esxI, (Putative ESAT-6 like protein) | 9.830 | 0.737 | Undecided | Globular | WXG100 (2.83e-08) |
| Rv3890c | esxC, (ESAT-6 like protein) | 9.920 | 0.800 | Undecided | Globular | COG4842[COG4842], Uncharacterized protein conserved in bacteria (1.65e-15) |
| Rv3337 | Conserved hypothetical protein | 13.180 | 0.698 | Undecided | Globular | Abhydrolase_6[pfam12697], Alpha/beta hydrolase family (5.08e-04) |
| Rv3067 | Conserved hypothetical protein | 13.930 | 0.768 | Undecided | Globular | Protein of unknown function (DUF732) pfam05305 (1.27e-19) |
| Rv3705c | Conserved protein | 22.360 | 0.689 | Undecided | Globular | PknH_C[pfam14032], PknH-like extracellular domain (6.95e-37) |
| Rv1115 | Possible exported protein | 24.050 | 0.665 | Undecided | Globular | None |
| Rv3717 | Conserved hypothetical protein | 24.840 | 0.698 | Undecided | Globular | MurNAc-LAA[cd02696], N-acetylmuramoyl-L-alanine amidase (8.86e-39) |
| Rv0590 | mce2B, (MCE-Family protein) | 29.160 | 0.685 | Undecided | Globular | MCE[pfam02470], mce related protein, ABC-type transport system, (1.78e-47) |
| Rv3207c | Conserved protein | 31.030 | 0.797 | Undecided | Globular | DUF3152[pfam11350], Protein of unknown function (DUF3152) (2.07e-107) |
| Rv0988 # | Possible conserved exported protein | 42.780 | 0.697 | Undecided | Globular | COG5621[COG5621], Predicted secreted hydrolase (2.01e-147) |
Notes:
*
Annotation according to TubercuList [42].
$
E-value from CDD search.
#
There was no evidence from the algorithms for ‘extracellular’ (claim) for Rv0988. Therefore it was declared ‘undecided’ because the TubercuList annotation reports this protein as exported protein.