Figure 6.

Metabolite fingerprinting of bran extracts using NMR and DI-ESI-MS datasets. Extracts from 5 day old T. hamatum bran inoculum were analysed by ¹H-NMR and direct infusion electrospray ionization mass spectrometry. Principal components analysis (PCA) of full unfiltered data at 95% confidence intervals was used to evaluate differences in chemistry between the bran extracts. PCA data showed clear separation of GD12 (black) and ΔThnag1::hph (red) from control bran extract green indicating both chemistries captured differences in the secreted metabolome. This unsupervised multivariate PCA analysis was performed using SIMCA-P 11.0, using mean-centered scaling.