Skip to main content
. 2013 Jul 30;7:25. doi: 10.3389/fnana.2013.00025

Figure 1.

Figure 1

Photomicrographs representing sections of the brainstem of GAD67-GFP knock-in mice in the DCN. Nissl staining in the CNC (A) showing the dorsal part (DCN) and the ventral part (VCN) (a in A). The DCN organized into a layered structure (b in A): the molecular layer (I), the fusiform cell layers (II) and the deep layer (III). Triple-labeled neurons for GFP/GABABR1/PKCγ were shown (B). Fluorescent photomicrographs showing the distribution of the GFP-labeled (green), GABABR1-positive (red) and PKCγ-positive (blue) cells in the molecular layer and fusiform cell layer of the DCN of the GAD67-GFP knock-in mice (C). The arrowheads indicate the triple-labeled neurons for GFP/GABABR1/PKCγ shown in (D). The GABABR1-immunoreactivities are located at the GFP-labeled GABAergic neurons which contain PKCγ (E, higher magnification areas, inserted panels in D). Scale bar: 500 μm in (Aa); 100 μm in (Ab); 10 μm in (B); 100 μm in (C); 30 μm in (D); 10 μm in (E).