Figure 3.

Photomicrographs representing sections of the brainstem of C57 mice in the DCN. Fluorescent images showing the GAD67-positive puncta are distributed densely in the molecular layer and fusiform cell layer of the DCN (A). In (B), the arrowheads indicate the triple-labeled neuron for GAD67/GABA_BR1/PKCγ (higher magnification areas, inserted panels in A). In (C), The arrowheads indicate that the GAD67-positive terminals made connections on the double-labeled neurons (higher magnification areas, inserted panels in B). Scale bar: 100 μm in (A); 30 μm in (B); 10 μm in (C).