Fig. 4.
Effect of ECFC-CM on PAEC in vitro angiogenesis. A: light microscopy shows that exposure to hyperoxia significantly decreases tube formation. Treatment with either term ECFC-CMRA or preterm ECFC-CM50 causes a striking increase in angiogenesis. B: quantification of tube formation by branch point in RA. All 4 types of ECFC-CM augmented tube formation. However, preterm ECFC-CM50 stimulated angiogenesis to a lesser degree than preterm ECFC-CMRA. C: branch point assessment of tube formation in hyperoxia. All 4 types of ECFC-CM augment in vitro angiogenesis. HUVEC-CMRA had no effect on tube formation. *P < 0.05, **P < 0.01, ***P < 0.001.