Fig. 9.
Chronic hypoxia upregulates mRNA and protein expression of calcium channel voltage-dependent L-type α1C-subunit (Cav1.2) and T-type α1H-subunit (Cav3.2) in PA but not in MA from mice. A: relative mRNA expression levels of Cav1.2, Cav2.1, Cav3.1, and Cav3.2 in PA (a) and MA (b) isolated from Nor (open bars, n = 6) and Hyp (solid bars, n = 5) mice. **P < 0.01 vs. Nor. c, Comparison of hypoxia-mediated changes in mRNA expression of Cav1.2, Cav2.1, Cav3.1, and Cav3.2 in MA (open bars) and PA (solid bars). **P < 0.01 vs. MA. B: a, immunohistochemical images (×60) showing Cav1.2 and Cav3.2 in PA (left) and MA (right) isolated from Nor (top) and Hyp (bottom) mice. b, Summarized data (means ± SE) showing mRNA expression level of Cav1.2 and Cav3.2 in PA and MA isolated from Nor (open bars; n = 8) and Hyp (solid bars; n = 8) mice. *P < 0.05 and **P < 0.01 vs. Nor. C: a, representative Western blot image of protein lysates from normoxic and hypoxic mouse lung tissues probed for Cav1.2 (top) and Cav3.2 (bottom). Normoxic and hypoxic samples were loaded on the same membrane. Brain tissues where probed as a positive control. b, Summarized data (means ± SE) showing protein expression level normalized to β-actin for Cav1.2 and Cav3.2 in normoxic and hypoxic mouse lung homogenates (n = 3, *P < 0.05 vs. Nor).