Fig. 1.

Pulmonary endothelial cells express the mammalian soluble adenylyl cyclase isoform 10 (AC10). A: using RT-PCR and specific primers, the AC10 transcript is detected in whole lung tissue and in pulmonary artery endothelial cells (PAECs) and pulmonary microvascular endothelial cells (PMVECs) B: AC10 protein is detected in both PAECs and PMVECs as 2 bands at ∼50 and 48 kDa using the R21 antibody, which recognizes an epitope around the second catalytic domain of AC10. There is greater expression of both AC10 isoforms in PMVECs compared with PAECs. Images are representative of at least three independent experiments.