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Western blot analysis of EMT markers E-cadherin and α-SMA after TGF-β1, IL-4 and IL-17A stimulation. The 16-HBE cells were serum-starved overnight and then stimulated with 10 ng/ml of TGF-β1, IL-4 and IL-17A alone or in combination for 72 h. The cells were next harvested for Western blot analysis of E-cadherin and α-SMA. β-actin was used for normalization, and the relative protein levels were presented as a ratio with control cells. The experiments were conducted with 3 replications.
