Figure 2.

Microarray data analysis. A: Selection of significantly altered genes. The normalized relative difference of gene expression following paclitaxel treatment from 0 to 24 h was calculated by SAM method. Left: for down-regulated genes, when false discovery rate (FDR) was fixed on 0.1%, cutoff value of the normalized relative difference was -2.25. Genes with relative difference lower than -2.25 were identified as significantly down-regulated genes. Right: for up-regulated genes, when FDR was fixed on 0.1%, cutoff value of the normalized relative difference was 1.95. Genes with relative difference higher than 1.95 were identified as significantly up-regulated genes. Middle: criterions above mentioned were applied to all genes on the microarray chip, significantly up-regulated genes were labeled with blue dots and significantly down-regulated genes were dotted with red color. B: Dynamic changes of gene expression patterns between paclitaxel and docetaxel treatment at two time stages. All genes were dotted by their normalized relative difference value between two stages: 0-24 h and 24-48 h.