Table 1.

Tumor cells in vivo cytogenetic evolution

Cell Source	Timeline	Clonal Cytogenetic Abnormalities	Interpretation
Tumor cells from seroma fluid	Original Tumor clones 4/18/12	45, XX [cp19] dup(X)(q11q28), +1, del(1)(q32), i(1)(q10), add(3)(p11), der(3)t(2;3)(p12;p26), +6, der(6)t(6;8)(q12;q21.3)x2, add(8)(q11.2), add(11)(q23), add(14)(p11.1), -15, -17, -20 80~91, idem [cp2]	An abnormal near-diploidy cell population (19/21) bearing a highly complex karyotype and a near-tetraploidy version (2/21) were detected in metaphase cells by conventional G-banding techniques. The 97.7% of this abnormal population showed negative ALK rearrangement with an ALK copy number change by interphase FISH techniques.
Cells from lymph node	Secondary Tumor Clones 10/19/12	34, XX [1]* +1, del(1)(q32)x2, +2, -3, der(3)t(2;3)(p12;p26), +6, der(6)t(6;8)(q12;q21.3)x2, add(9)(p22), -10, -12, -12, -13, -14, -15, add(15)(p11.2), -16, add(16)(q11.2), -17, -18, -20, -20, -21, -21, -22 [1] 78-79, XX [cp3]* +X, +X, +1, +1, del(1)(q32)x2, i(1)(q10), +2, add(3)(p11), add(4)(q21), +6, +6 der(6)t(6;8)(q12;q21.3)x2, -8, add(9)(p22)x1-3, -10, add(10)(p13), add(11)(q23), +14, -15, add(15)(p11.2), +16, add(16)(q11.2)x2, -17, -17, -18, +19, -20, -22, i(22)(q10), +mar1-2 46, XX [16]	Two related (variant) abnormal cell populations bearing highly complex karyotypes were detected in 4/20 metaphase cells. Of the four, one is a hyperhaploidy with chromosome count of 34, while the remaining three contained a hypertriploidy with chromosome count of 78-79. Common clonal cytogenetic changes observed among these abnormal cells indicate ploidy variants (or evolution) of a stemline or primary cell population.

^*Bold-highlighted cytogenetic abnormalities are shared by original and secondary tumor clones.