. 2013 May 1;305(1):R31–R41. doi: 10.1152/ajpregu.00402.2012

Table 2.

Effects of SS-rBF administration of CGS-21680 on c-Fos expression in GABAergic neurons of the MnPN and VLPO

Area	Sleep-Wake Condition	Treatment (n = 7)	# GAD-IR Neurons	# Fos-IR Neurons	# Dual GAD/Fos-IR Neurons
Rostral MnPN	UD	Vehicle	92.6 ± 3.5	17.4 ± 3.2	6.7 ± 1.97
		8 nmol CGS	84.3 ± 3.1	18.6 ± 2.6	12.9 ± 2.0
		t-test	t₍₁₂₎ =1.75 P = 0.10	t₍₁₂₎ = −0.30 P = 0.76	t₍₁₂₎ = −2.20 P < 0.05
	SD	Vehicle	94.8 ± 2.3	20.2 ± 1.98	7.1 ± 1.12
		8 nmol CGS	90.0 ± 4.4	21.9 ± 3.2	11.0 ± 1.1
		t-test	t₍₁₂₎ = 0.96 P = 0.35	t₍₁₂₎ = −0.42 P = 0.67	t₍₁₂₎ = −2.49 P < 0.05
Caudal MnPN	UD	Vehicle	73.5 ± 0.8	12.4 ± 2.7	3.7 ± 0.5
		8 nmol CGS	69.1 ± 2.9	11.8 ± 1.9	8.5 ± 1.5
		t-test	t₍₁₂₎ =1.40 P = 0.18	t₍₁₂₎ =0.20 P = 0.84	t₍₁₂₎ = −2.91 P < 0.05
	SD	Vehicle	69.1 ± 2.9	11.8 ± 1.9	5.4 ± 0.96
		8 nmol CGS	76.2 ± 1.5	18.0 ± 1.8	9.5 ± 0.76
		t-test	t₍₁₂₎ =1.11 P = 0.28	t₍₁₂₎ =0.95 P = 0.35	t₍₁₂₎ = −3.29 P < 0.01
Core VLPO	UD	Vehicle	44.4 ± 3.0	7.6 ± 1.0	7.2 ± 1.6
		8 nmol CGS	41.9 ± 3.2	9.1 ± 1.6	11.5 ± 1.0
		t-test	t₍₁₂₎ =0.56 P = 0.58	t₍₁₂₎ = −0.80 P = 0.43	t₍₁₂₎ = −2.25 P < 0.05
	SD	Vehicle	46.5 ± 2.4	8.8 ± 1.4	6.8 ± 0.5
		8 nmol CGS	43.0 ± 2.8	10.9 ± 2.3	10.1 ± 0.87
		t-test	t₍₁₂₎ =0.94 P = 0.36	t₍₁₂₎ = −0.79 P = 0.44	t₍₁₂₎ = −3.28 P < 0.01
Extended VLPO	UD	Vehicle	101.1 ± 9.3	12.1 ± 2.0	10.7 ± 1.2
		8 nmol CGS	86.3 ± 4.2	15.9 ± 1.6	24.0 ± 1.4
		t-test	t₍₁₂₎ =1.43 P = 0.17	t₍₁₂₎ = −1.49 P = 0.16	t₍₁₂₎ = −7.13 P < 0.01
	SD	Vehicle	101.2 ± 4.7	12.9 ± 1.9	10.3 ± 1.8
		8 nmol CGS	86.0 ± 5.56	16.6 ± 2.36	19.5 ± 1.5
		t-test	t₍₁₂₎ =2.08 P = 0.059	t₍₁₂₎ = −1.20 P = 0.25	t₍₁₂₎ = −3.94 P < 0.01

All values are expressed as means ± SE (t-test). UD indicates rats that were permitted undisturbed sleep during 2 h postinjection. SD indicates rats subjected to 2 h of sleep deprivation postinjection.