Table 3.

Effects of intracerebroventricular infusion of ZM-241385 during sleep deprivation on c-Fos expression in GABAergic neurons of the MnPN and VLPO during 2 h of recovery sleep

Area	Treatment (n = 6)	# GAD-IR Neurons	# Fos-IR Neurons	# Dual GAD/Fos-IR Neurons
Rostral MnPN	Vehicle	85.7 ± 1.8	27.5 ± 2.3	14.9 ± 1.2
	5 nmol ZM	88.9 ± 3.1	24.4 ± 2.4	10.4 ± 2.1*
	25 nmol ZM	90.7 ± 2.2	22.7 ± 2.8	8.3 ± 1.3**
	ANOVA (Holm-Sidak test)	F(2,15) =1.05 P = 0.37	F(2,15) =0.97 P = 0.40	F(2,15) =7.79 P < 0.01
Caudal MnPN	Vehicle	70.1 ± 3.3	17.5 ± 2.3	8.5 ± 0.8
	5 nmol ZM	71.2 ± 3.1	16.9 ± 2.0	6.1 ± 0.7*
	25 nmol ZM	75.2 ± 2.8	14.1 ± 1.9	5.2 ± 0.2**
	ANOVA (Holm-Sidak test)	F(2,15) =0.90 P = 0.42	F(2,15) =0.71 P = 0.50	F(2,15) =7.00 P < 0.01
Core VLPO	Vehicle	38.9 ± 2.7	7.1 ± 1.2	9.5 ± 1.2
	5 nmol ZM	40.5 ± 1.9	7.1 ± 1.2	6.6 ± 0.7*
	25 nmol ZM	42.8 ± 2.9	4.1 ± 0.9	5.8 ± 0.6**
	ANOVA (Holm-Sidak test)	F(2,15) =0.70 P = 0.51	F(2,15) =2.35 P = 0.12	F(2,15) =4.86 P < 0.05
Extended VLPO	Vehicle	80.3 ± 6.2	13.5 ± 2.4	18.6 ± 2.7**
	5 nmol ZM	87.1 ± 2.7	13.4 ± 2.4	10.9 ± 1.9**
	25 nmol ZM	91.5 ± 2.5	9.3 ± 1.5	9.0 ± 0.5**
	ANOVA (Holm-Sidak test)	F(2,15) =1.90 P = 0.18	F(2,15) =1.26 P = 0.31	F(2,15) =7.19 P < 0.01

All values are expressed as means ± SE.

^*P < 0.05,

^**P < 0.01 level of significance (Holm-Sidak test).