Fig. 3.
Effects of S100A1 on wild-type and RyR2-L3591D single-channel activities. Top: single RyR2 channels isolated from hearts were recorded in the presence of 0.2 μM (A) and 2 μM (B) cis cytosolic Ca2+ as described in Fig. 2 in the absence of S100A1 (top) and on the subsequent addition of 0.3 μM (middle) and 1 μM (bottom) cis S100A1. Bottom: mean ± SE Po of number of recordings indicated in parentheses, normalized to control (−S100A1). *P < 0.05, **P < 0.001 compared with control (no S100A1 added).