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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Immunol. 2013 Jun 24;191(3):1404–1412. doi: 10.4049/jimmunol.1300856

FIGURE 2.

FIGURE 2

Aggrecan degradation products induced by hTryptase-β possess MMP- but not ADAMTS-dependent neoepitopes. A-C, Immunoblots of conditioned media from devitalized femoral head explants cultured in media alone, rhTryptase-β (15 μg/ml), trypsin (5 μg/ml), or live cartilage explants stimulated to induce aggrecanase activity using rhIL-1β (10 ng/ml), probed with antibodies that detect the aggrecanase-specific ALG neoepitope (panel A), the MMP-specific FFGVG neoepitope (panel B), or the MMP-specific DIPEN neoepitope (panel C). D and E, Immunohistochemistry with anti-DIPEN antibody in sections of femoral head explants cultured in the absence (D) or presence of 15 μg/ml rhTryptase-β (E). Isotype controls for immunohistochemistry are inset in the panels; scale bars = 50 μm.