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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Immunol. 2013 Jun 24;191(3):1404–1412. doi: 10.4049/jimmunol.1300856

FIGURE 3.

FIGURE 3

Human and mouse MC tryptases induce MMP activity in femoral head explants. A-F, Aggrecan-derived GAG released into the culture media from femoral head explants (panels A, B, and C) and MMP enzymatic activity in extracts from femoral head explants (panels D, E, and F) following incubation with 15 μg/ml rhTryptase-β (panels A and D), 15 μg/ml rmMCP-6 (panels B and E), or lysates isolated from 105 mMCP-6+/+ or mMCP-6−/− PMCs (panels C and F). Culture of femoral heads with media alone or 5 μg/ml trypsin represent negative and positive controls for optimal GAG release respectively. *, p < 0.05; **, p < 0.01; ***, p < 0.001; error bars represent S.E.M.; n = 3.